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How to Do Serum Free Media Cell Culture?

How to Do Serum Free Media Cell Culture? - Cell Biology and Cell Culture

How to Do Serum Free Media Cell Culture? - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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Old 08-27-2007, 01:36 PM
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Default How to Do Serum Free Media Cell Culture?



Hello all,
I am confused.

How can i do serum free media cell culture?

thanks
Kiki
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Old 10-11-2007, 08:33 PM
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Default Re: How to Do Serum Free Media Cell Culture?

Quote:
Originally Posted by kiki06 View Post
Hello all,
I am confused.

How can i do serum free media cell culture?

thanks
Kiki
We weaned cell cultures off serum over a 5-7 day period. Our first time doing this. It worked very well, going from 100% usual serum to 75% to 50% to 25% to 0%. We even asked ourselves if going from normal serum media to serum free media in one step could have been possible, but we didn't bother to investigate. The cells seemed impervious to the media changes, no morphological, dying off, no slower replication absolutely no difference. They lived and divided for a month until we stopped culturing and collected the cells.
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