| | Re: Cell Culture: Why FBS fetal bovine serum?
Regarding your question about FBS, in our lab, we use FBS but not BS (bovine serum) when:
(1) If we want to investigate the immune system or reaction in vitro by using cells, such as to detect the change of level of cytokines or T, B cells system in response to some treatments, we use FBS. Because BS has a lot of factors that can influence our treatments. And as far as I know, we get the FBS before the new born cattle get milk for their moms which is a major way for new born babies to get antibodies.
Of course you can also heat the BS to degrade those potential factors.
(2) When we culture some sensitive cells, such as BT474, BT20, MDA-MB-231, MCF7 which needs much more nourishment, we use FBS but not BS, because the former is much more nutritous than the latter.
Hope those can help you a little bit.