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Cell Trypsinization Protocol

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saving-the-cell-pellet-cell-culture It is very easy to accidentally suck up your cells in this step along with your media. Tilt the tube as shown and do not let the pipette get too close to the pellet. Saving the Cell Pellet Cell Culture Methods and Protocols. From Youtube user be115

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Cell Trypsinization Protocol

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.

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#1 (permalink)

08-27-2007, 12:13 PM

oBWhat

Graduate Student

Join Date: May 2006

Posts: 120

Blog Entries: 1

Cell Trypsinization Protocol

Hello everyone,

does anyone have a careful method of trypsinizing primary cell cultures or similar cultures that are very sensitive?

I hate hitting the flasks to get my cells off.

Dont want to disturb my cells too much

Cheers
ObWhat

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Cell Trypsinization Protocol

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.