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08-27-2007, 12:12 PM
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 Cell Lysis for Enzyme Activity Hello all, I am back with a question some expert may know.  I want a lysis method that does not denature the proteins much. I know there are french presses for lysis using pressure etc. but what is really the best way to lyze cells for analysis later of enzyme activity? this requires non-denaturing conditions and minimal disruption of the protein secondary and tertiary structure. Any ideas?  cheers ObWhat        |
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