How Long Cell Storage in -80C Freezer Time?

kiki06 · #1 (**permalink**) 08-27-2007, 12:07 PM

Hello everyone,
I am freezing aliquots of cells in the -80C freezer.

How long (Months? Years?) can cells be stored in the -80C?

I will be using these cells to run westerns and possibly run on a 2-D gel for phosphorylation analysis.

thanks
Kiki

IoDiNE · #2 (**permalink**) 08-27-2007, 12:11 PM

Hi
Well according to my experience at -80 C, The cells can be propagated again if culture them within 6 months. but it also depends on type of freezing media you are using for that, but at -80 it is usually good to use the cells within 6 months.
regards
aftab

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How Long Cell Storage in -80C Freezer Time?

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