Low cell growth

JinJinO · #1 (**permalink**) 08-27-2007, 12:00 PM

HELP!
I have the big problem with growth of stable line HEK+plasmid (G418,hygromycin) cells that we got from our co-laborators. It wasn't mycoplasme (I've tested).
Do you have some ideas about it?

danfive · #2 (**permalink**) 08-27-2007, 12:02 PM

"Recommended concentration for the selection of resistant cells is 25-1000 mg/ml. Commonly used concentrations for selection are 200 mg/ml for mammalian cells, 20-200 mg/ml for plant cells & bacteria cells and 200-1000 mg/ml for fungi. Your optimum concentration should be tested experimentally."
from hygromycin.com

Check your plates for cell adhesion, if you have a lot of dead floating cells wash them off and keep the adhered cells, add fresh media. Also try titrating the hygromycin antibiotic from low concentration to higher. Like start low then let them grow overnight, then boost the antibiotic in several steps the next day. By the time you pass cells in 3-4 days you should only have live resistant cells.

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
No cell growth with antibiotic plates	hdraheim	Molecular Biology Techniques	1	03-10-2008 10:08 PM
Yeast Cell Protein Complex Study	molecule2005	Science News and Views	0	11-07-2007 03:37 AM
Mouse Embryo Model for Stem Cell Research	moleculardude	Stem Cell Forum	0	07-05-2007 01:18 AM
A human colon cancer cell capable of initiating tumour growth in immunodeficient mice	admin	Science News and Views	0	12-05-2006 07:06 AM
Cell Passage Number for Cell Culture	kiki06	Cell Biology and Cell Culture	1	09-06-2006 10:14 AM

Member Login

Low cell growth

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.