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Non-Adherent Cell Line Attachment

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saving-the-cell-pellet-cell-culture It is very easy to accidentally suck up your cells in this step along with your media. Tilt the tube as shown and do not let the pipette get too close to the pellet. Saving the Cell Pellet Cell Culture Methods and Protocols. From Youtube user be115

Cell Biology and Cell Culture

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Non-Adherent Cell Line Attachment

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.

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08-27-2007, 11:37 AM

dnamolecule dnamolecule is offline

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Non-Adherent Cell Line Attachment

Hi everyone,
I have a problem. A cell line that I am maintaining (U-937) is now attaching to the flask very strongly.

I am using 10% FBS

Why is this occurring?
thanks

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Non-Adherent Cell Line Attachment

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.