Endothelial Permeability Assay

kiki06 · #1 (**permalink**) 07-09-2007, 12:04 PM

Hello,
Does anyone have a method for endothelial permeability assays.

Some papers use 3um costar transwell plates for HUVEC.

However, I want to know a whole protocol which is well detailed because I am new in this area of research.

Thank you

katherine_ft · #2 (**permalink**) 07-24-2007, 02:02 AM

Our lab has worked on HUVEC before. What they did is "cell invasion assay". I am not sure if that is the same method as yours. The principle is as follow:
HUVEC cells were grown in the insert well with drugs. after period of time incubation, cells would go through the membrance and to the bottom of the insert well. A cell staining will then be carried out. Quantifcation by fluorescent imaging.

Thread Tools
Show Printable Version Email this Page
Display Modes
Linear Mode Switch to Hybrid Mode Switch to Threaded Mode

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Filter Assay for the Detection of Yeast Colonies Beta-Galactosidase	oBWhat	Protocols and Methods Forum	3	07-29-2008 03:28 AM
Caspase-3 Cleavage Assay	pipette_man	Protein Science	0	04-15-2008 10:51 AM
TUNEL assay Protocol	oBWhat	Apoptosis, Autophagy, and Necrosis Forum	0	06-29-2007 08:34 PM
Nuclear run-on assay (contribution of elongation)	Hetty	RNA Techniques Forum	0	06-17-2007 12:04 PM
Flow Chamber Assay Protocol	moleculardude	Cell Biology and Cell Culture	0	11-29-2006 12:02 AM

Member Login

Endothelial Permeability Assay

Cell Biology and Cell Culture

Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.