Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture
Register Search Today's Posts Mark Forums Read

Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


G418 selection of stable cell

G418 selection of stable cell - Cell Biology and Cell Culture

G418 selection of stable cell - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-07-2006, 08:42 PM
oBWhat's Avatar
Graduate Student
Points: 3,826, Level: 41 Points: 3,826, Level: 41 Points: 3,826, Level: 41
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: May 2006
Posts: 124
Thanks: 0
Thanked 7 Times in 7 Posts
Default G418 selection of stable cell



How can I conduct a G418 selection to select for stable cell lines? I am trying to integrate a plasmid sequence containing G418 resistance and a promoter driving the expression of a gene X.

What is the protocol / general procedure for this? any tips to do this? thanks
Reply With Quote
  #2  
Old 08-08-2006, 07:56 PM
Pipette Filler
Points: 2,169, Level: 29 Points: 2,169, Level: 29 Points: 2,169, Level: 29
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jun 2006
Posts: 19
Thanks: 0
Thanked 1 Time in 1 Post
Default Re: G418 selection of stable cell

You need to get your hands on some information first. See what pubmed articles did G418 selection for your cell line of interest. what concentrations did they use? how long they selected?

using these you can figure out a few things... then just make a concentration curve and find your LD50 that kills 50% of your cells... after a certain time you can get selected cells growing. these should have your vector of interest integrated.
Reply With Quote
Reply

Tags
cell , g418 , selection , stable


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
Human Cytome Project - an idea - Update 19 April 2005 Peter Van Osta Cell Biology and Cell Culture 1 06-01-2009 02:17 PM
A Human Cytome Project - an idea - Update 14 March 2005 Peter Van Osta Cell Biology and Cell Culture 0 03-14-2005 02:27 PM
Human Cytome Project - Update 6 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 0 01-06-2005 11:18 AM
Tissue Engineering & Stem Cell Research 2005 Andrew Oliver Protocols and Methods Forum 0 12-02-2004 11:38 PM


All times are GMT. The time now is 09:52 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.16739 seconds with 16 queries