Antifungal compound

[aftabac]

Hi
Sometime we got fungal contemination in cell culture flasks, so what is the best antifungal compound used in cell culture media to avoid cell contemination and what is its concentration used.

best regards
aftab ahmad

[moleculardude]

Dear Aftab,

hello how are you?

That is a good question. We use an Antibiotic / Antimycotic (anti-fungal) solution made up of Penicillin-Streptomycin.

Amphotericin B (an antimycotic) would be an antifungal. We add this to all our cell culture solutions and never have problems with fungus in cell culture.

Cheers!

[aftabac]

Hi
me fine thanks
and thanks a lot for your guidence


best regards
aftab ahmad

[aftabac]

Well i think that streptomycin has antibacterial action not the antifungal action, so which drug we must use as an antifungal agent in cell culture media.

regards
aftab

[moleculardude]

Hello Aftab,

Sorry! Actually antimycotics (anti-fungals) include Polyene antimycotics such as:
Amphotericin B
Natamycin 46
Rimocidin
Filipin 35 Carbons binds to cholesterol (toxic)
Nystatin

If you purchase an antibiotic / antimycotic cell culture mix (or make it yourself) you should be ok!

Cheers!
Moleculardude

[guynextdoor84]

hi, i have fungal like contamination, can anyone help me to identify what are them? and i saw some spider web like, some spore like, are they all different or one species of mold that are at different stage?
here are the pictures:





Please help me. Any suggestion to help eradicate these bugs and prevent from spreading to other reagents and cell lines are more than welcome. Please reply to [Only registered users see links. ]