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Antifungal compound

Antifungal compound - Cell Biology and Cell Culture

Antifungal compound - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


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  #1  
Old 03-11-2007, 02:28 PM
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Default Antifungal compound



Hi
Sometime we got fungal contemination in cell culture flasks, so what is the best antifungal compound used in cell culture media to avoid cell contemination and what is its concentration used.

best regards
aftab ahmad

Last edited by moleculardude; 03-11-2007 at 02:36 PM.
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Old 03-11-2007, 02:36 PM
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Smile Re: Antifungal compount

Dear Aftab,

hello how are you?

That is a good question. We use an Antibiotic / Antimycotic (anti-fungal) solution made up of Penicillin-Streptomycin.

Amphotericin B (an antimycotic) would be an antifungal. We add this to all our cell culture solutions and never have problems with fungus in cell culture.

Cheers!

Last edited by moleculardude; 03-14-2007 at 08:09 PM. Reason: Sorry mixed up!
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Old 03-11-2007, 06:00 PM
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Default Re: Antifungal compound

Hi
me fine thanks
and thanks a lot for your guidence


best regards
aftab ahmad
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Old 03-14-2007, 05:48 PM
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Default Re: Antifungal compound

Well i think that streptomycin has antibacterial action not the antifungal action, so which drug we must use as an antifungal agent in cell culture media.

regards
aftab
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Old 03-14-2007, 08:09 PM
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Default Re: Antifungal compound

Hello Aftab,

Sorry! Actually antimycotics (anti-fungals) include Polyene antimycotics such as:
Amphotericin B
Natamycin 46
Rimocidin
Filipin 35 Carbons binds to cholesterol (toxic)
Nystatin

If you purchase an antibiotic / antimycotic cell culture mix (or make it yourself) you should be ok!

Cheers!
Moleculardude
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Old 06-23-2009, 04:56 PM
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Default Re: Antifungal compound

hi, i have fungal like contamination, can anyone help me to identify what are them? and i saw some spider web like, some spore like, are they all different or one species of mold that are at different stage?
here are the pictures:





Please help me. Any suggestion to help eradicate these bugs and prevent from spreading to other reagents and cell lines are more than welcome. Please reply to [Only registered users see links. ]
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