Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Cell Biology and Cell Culture
Register Search Today's Posts Mark Forums Read

Cell Biology and Cell Culture Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


DMEM Media

DMEM Media - Cell Biology and Cell Culture

DMEM Media - Cell Biology Forum. Cell Culture Forum. Post and ask questions about cell culturing, cell lysis, cell transfection, cell growth, and cell biology.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 03-11-2007, 02:04 PM
aftabac's Avatar
Nobel Laureate
 
Join Date: Jun 2006
Location: Pakistan
Posts: 570
Thanks: 5
Thanked 41 Times in 34 Posts
Default DMEM Media



Hi
Well first we buy already prepared DMEM media from supplier but now we are getting powdered DMEM Media that do not contain Sodium Bicarbonate, so please guide me whether Sodium bicarbonate is necessary in DMEM composition and what is its role and secondly if it is necessary then at what concnetration it should be added.


best regards
aftab ahmad
Reply With Quote
  #2  
Old 03-20-2007, 11:58 AM
aftabac's Avatar
Nobel Laureate
 
Join Date: Jun 2006
Location: Pakistan
Posts: 570
Thanks: 5
Thanked 41 Times in 34 Posts
Default Re: DMEM Media

Hi
i have successfully prepare the meida form powder and the cells are also grwoing very well in new media, i just added sodium bicarbonate in the media and then adjust the pH and then filter sterlize it.

regards
aftab ahmad
Reply With Quote
Reply

Tags
dmem , media


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
mouse hepatocytes isolation amicia Cell Biology and Cell Culture 39 05-11-2012 11:52 AM
Production of Monoclonal Antibodies - Technique in Mouse Cellbiogal Antibody Forum 2 01-08-2009 10:52 PM
Media left at 37 C O/N? Kamalian, Laleh Protocols and Methods Forum 0 02-10-2008 08:15 PM
DMEM media Pandu Cell Biology and Cell Culture 0 11-18-2007 11:27 PM
5x met/cys media Lisa Imboden Protocols and Methods Forum 2 05-16-2007 12:54 PM


All times are GMT. The time now is 06:03 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12016 seconds with 16 queries