Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > DNA Techniques > cDNA Forum
Register Search Today's Posts Mark Forums Read

cDNA Forum cDNA Forum. Discuss complementary DNA synthesis, cDNA libraries, and cDNA cloning in this forum.


cDNA storing

cDNA storing - cDNA Forum

cDNA storing - cDNA Forum. Discuss complementary DNA synthesis, cDNA libraries, and cDNA cloning in this forum.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-08-2011, 02:59 PM
Pipette Filler
Points: 34, Level: 1 Points: 34, Level: 1 Points: 34, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2011
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default cDNA storing



Hi everybody

I have problems with cDNA storing, i have tried -70 and -20, but when im gonna work with this samples i dont get any results...

I need help..!!!
Reply With Quote
  #2  
Old 02-08-2011, 06:54 PM
admin's Avatar
Administrator
 
Join Date: Nov 2005
Posts: 1,418
Thanks: 883
Thanked 68 Times in 58 Posts
Default Re: cDNA storing

Hi there
what are you trying to do with your cDNA?

You may have to look at how you are storing it:

A) temperature
B) Buffer and salts used as final storing - will this affect your enzymatic reactions?
ie your cDNA could be there but you cannot get anything from it
C) is your cDNA degraded or poor quality to begin with?
Reply With Quote
  #3  
Old 02-08-2011, 07:20 PM
luisillo's Avatar
M.D/Ph.D
Points: 3,734, Level: 40 Points: 3,734, Level: 40 Points: 3,734, Level: 40
Activity: 99% Activity: 99% Activity: 99%
 
Join Date: Jul 2010
Location: Mexico
Posts: 348
Thanks: 19
Thanked 96 Times in 88 Posts
Default Re: cDNA storing

You should try working with your cDNA right away to make sure it IS there. Once you're certain that you succesfully obtained cDNA, you should test the storage conditions. Since you should have the resulting cDNA diluted on DEPC treated water (or something nuclease free), and since it is DNA, it should be safe stored at -20C, don't know how much time though.
Reply With Quote
  #4  
Old 08-11-2011, 09:13 AM
Volunteer
Points: 231, Level: 4 Points: 231, Level: 4 Points: 231, Level: 4
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Aug 2011
Location: Malaysia
Posts: 36
Thanks: 0
Thanked 14 Times in 13 Posts
Default Re: cDNA storing

Maybe its not bout the storage, perhaps its reverse transcription problem.
you should do a quality check on your cDNA right after RT, simply by conducting a RT-PCR with housekeeping genes.
Reply With Quote
Reply

Tags
cdna , storing


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Help needed on aminoallyl-labeled cDNA palver Molecular Biology Articles and Protocols 0 01-07-2009 08:40 AM
cDNA Synthesis and Amplification of POLY(A) mRNA by RT-PCR Protocol domba Real-Time PCR and Quantitative PCR Forum 3 09-09-2008 02:48 PM
Commercial cDNA or cdna Synthesized Random hemer for Transfection bhunni DNA Techniques 0 09-01-2007 07:12 AM
cDNA precipitation without coprecipitation of primers Simone Marker Protocols and Methods Forum 1 03-05-2007 08:36 PM
labelled cDNA stability? Claire Protocols and Methods Forum 3 05-24-2004 07:38 PM


All times are GMT. The time now is 04:59 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.12965 seconds with 16 queries