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| cDNA Forum cDNA Forum. Discuss complementary DNA synthesis, cDNA libraries, and cDNA cloning in this forum. |
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07-23-2008, 11:53 AM
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 cDNA Synthesis Problem Hello i am having problems synthesizing full length cDNAs. what could be the problem? I am extracting RNA from cells then using Reverse transcriptase to get the RNA to cDNA however when i clone the cDNA into plasmids, the ones i am looking for are not there or short. plz help        |
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07-23-2008, 07:49 PM
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 Re: cDNA Synthesis Problem Improve the quality of your RNA and the problem should go away. I use Trizol, but a good kit can give you even better results. Also try to use fresh RNA since it will degrade, even if frozen. |
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07-29-2008, 12:41 PM
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| Re: cDNA Synthesis Problem just use GTC or any kit for rna isolation and do the RT. check ur cdna synthesis protocol u have to denature rna with oligo dt, cool it and then add dntp, buffer and enzyme and keep at 42 for 1hr u wil get it. The quality of rna should be good |
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