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Translating cDNA Sequences into Protein Sequence?

Translating cDNA Sequences into Protein Sequence? - cDNA Forum

Translating cDNA Sequences into Protein Sequence? - cDNA Forum. Discuss complementary DNA synthesis, cDNA libraries, and cDNA cloning in this forum.


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Old 07-20-2008, 05:18 AM
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Default Translating cDNA Sequences into Protein Sequence?



Hi didnt know where to post this as its mainly a bioinformatics question.

I currently have a cDNA sequence and would like to know how I can translate it into protein sequence.

I know there is a ORF finder here:[Only registered users see links. ]

but The cDNA sequence is not in FASTA format So how can I use ORF finder and translate it into protein?

thanks
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Old 07-20-2008, 05:19 AM
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Default Re: Translating cDNA Sequences into Protein Sequence?

Hello there,
what format is your cDNA in? You can either convert it into FASTA or find the FASTA at the NCBI pubmed if you do a nucleotide search for your cDNA


If it's short you could translate it also by hand. There are many online tools for going from DNA to protein here:
Http://ca.expasy.org/tools/
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Old 02-03-2009, 09:22 AM
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Default Re: Translating cDNA Sequences into Protein Sequence?

Check the screenshot attached, if it's suitable for your needs you can get have functionality with open source UGENE tool.
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Old 07-27-2009, 02:12 PM
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Default Re: Translating cDNA Sequences into Protein Sequence?

hi everyone, am new to this form.
I went to this forum and found really intresting and its helping out lot. You guys doing gr8 job.

I have a problem with the nucleotide sequence, hope some of you guys out there can help me out. I have two nucleotide sequence like A' of bacteria A and B' of bacteria B. these genes are 98% homologous, when i find ORFs in these sequence i get 1 ORF of nuceleotide A' and 2 ORF of nucleotide B'. The two ORF of nucleotide encode different genes so does the single ORF. The only difference between these nucleotide sequence is formation of ORFs. Whats the cause, is it some frameshift mutation if not than what. Maximum information would be helpful.

Thanks
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