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#1
09-11-2009, 11:20 AM
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 Single worm reverse-transcriptase (RT) PCR Dear colleagues, I'm advised that putative positives from RNA-interference (RNAi) experiments (using feeding strains from Geneservice {Fraser et al [2000]. Nature 408 (6810) 325-30}) can be checked by RT-PCR. This requires reverse transcriptase reaction on single worms. Single worm PCR lysis buffer doesn't seem amenable to reverse transcriptasereaction {?} Does anyone have a method/protocol for single worm RT-PCR ? G. Joshua. G.W.P.Joshua London School of Hygiene and Tropical Medicine Dept. Infectious and Tropical Diseases, Keppel St., London WC1E 7HT. Tel: (+44) (0)20 7927 2028 FAX: (+44) (0)20 7637 4314 E-mail: [Only registered users see links. ]  |
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| pcr , reversetranscriptase , single , worm |
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