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Single worm reverse-transcriptase (RT) PCR

Single worm reverse-transcriptase (RT) PCR - C Elegans Forum

Single worm reverse-transcriptase (RT) PCR - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


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Old 09-11-2009, 11:20 AM
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Default Single worm reverse-transcriptase (RT) PCR



Dear colleagues,
I'm advised that putative positives from RNA-interference (RNAi) experiments (using feeding strains from Geneservice {Fraser et al [2000]. Nature 408 (6810) 325-30}) can be checked by RT-PCR. This requires reverse transcriptase reaction on single worms.
Single worm PCR lysis buffer doesn't seem amenable to reverse transcriptasereaction {?}
Does anyone have a method/protocol for single worm RT-PCR ?
G. Joshua.


G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
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