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C Elegans Forum C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


Real Time PCR

Real Time PCR - C Elegans Forum

Real Time PCR - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


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  #1  
Old 01-08-2009, 12:11 PM
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Default Real Time PCR



Dear colleagues,
Is there a consensus on best/good housekeeping gene in C. elegans to use
to normalise against in Real Time PCR?
Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but
find large differences in RNAs from two worm populations (in all replicates).
Best regards,
G. Joshua.


G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel: (+44) (0)20 7927 2028
FAX: (+44) (0)20 7637 4314
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Old 10-17-2009, 02:15 PM
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Default Re: Real Time PCR

act-1 is OK
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Old 10-17-2009, 02:16 PM
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Default Re: Real Time PCR

David Hoogewijs et al. 2008.
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