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#1
01-08-2009, 11:11 AM
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 Real Time PCR Dear colleagues, Is there a consensus on best/good housekeeping gene in C. elegans to use to normalise against in Real Time PCR? Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but find large differences in RNAs from two worm populations (in all replicates). Best regards, G. Joshua. G.W.P.Joshua London School of Hygiene and Tropical Medicine Dept. Infectious and Tropical Diseases, Keppel St., London WC1E 7HT. Tel: (+44) (0)20 7927 2028 FAX: (+44) (0)20 7637 4314 E-mail: [Only registered users see links. ]  |
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