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Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum - Real Time PCR

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01-08-2009 11:11 AM

Real Time PCR

Dear colleagues,
Is there a consensus on best/good housekeeping gene in C. elegans to use
to normalise against in Real Time PCR?
Have tried act-3 (see Park et al (2007) J Mol Biol 372 (2), 331-340) but
find large differences in RNAs from two worm populations (in all replicates).
Best regards,
G. Joshua.

G.W.P.Joshua
London School of Hygiene and Tropical Medicine
Dept. Infectious and Tropical Diseases,
Keppel St.,
London WC1E 7HT.
Tel: (+44) (0)20 7927 2028
FAX: (+44) (0)20 7637 4314
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vividlily

10-17-2009 02:15 PM

Re: Real Time PCR

act-1 is OK

vividlily

10-17-2009 02:16 PM

Re: Real Time PCR

David Hoogewijs et al. 2008.

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