Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > C Elegans Forum
Register Search Today's Posts Mark Forums Read

C Elegans Forum C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


Advice regarding long-term liquid culture of C. elegans transgenics

Advice regarding long-term liquid culture of C. elegans transgenics - C Elegans Forum

Advice regarding long-term liquid culture of C. elegans transgenics - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-21-2008, 11:10 PM
Julie-Anne Fritz
Guest
 
Posts: n/a
Default Advice regarding long-term liquid culture of C. elegans transgenics



Dear Worm breeders,

We have generated a large number of transgenic worms in an unc-119
background using microparticle bombardment. We need large numbers of
these transgenics and have been growing them in liquid culture to
reach the density required. A few months ago, these strains were
growing really well with a quick generation time. More recently, the
same strains have become extremely slow growing and have not been
reaching the densities needed.

We have tried starting fresh cultures from frozen stocks, making
fresh culture reagents etc, but nothing as improved the growth of
these strains. The same strains have also not grown well when we have
put them on solid media.

Has anyone had any experience with the long-term liquid culture of
transgenics?? Is it perhaps the time of year, as it is now winter in
the Southern hemisphere!

Any help is appreciated.

Thanks in advance.

Julie-Anne


Julie-Anne Fritz, Ph.D.
Nematode Molecular Genetics Group
The School of Biochemistry & Molecular Biology
Faculty of Science
The Australian National University
Canberra, A.C.T., Australia 0200

Ph(office): + 61 2 6125 4941
Ph(lab): + 61 2 6125 5012
Fax: + 61 2 6125 0313
[Only registered users see links. ]




Reply With Quote
Reply

Tags
advice , culture , elegans , liquid , longterm , transgenics


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Politics And Cannibalism? Introducing The Dourties, Chelsea, Bill, Hillary, Barrack Obama, George Bush, Jr., And All Of Capital Hill! jon_johnfrancisayres@yahoo.com Microbiology Forum 0 10-06-2007 05:59 AM
c. elegans liquid cultures Jimmy Yeh C Elegans Forum 0 08-07-2003 01:09 PM


All times are GMT. The time now is 09:05 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13241 seconds with 16 queries