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collagenase treatment for staining

collagenase treatment for staining - C Elegans Forum

collagenase treatment for staining - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


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Old 01-12-2006, 10:03 PM
gsliwoski@mclean.harvard.edu
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Default collagenase treatment for staining



Hi, I'm trying to compile a protocol for Indirect Immunofluorescence (GFP)
for GABA in c elegans. I'm under the impression that collagenase treatment
is required for this anti-sera. However, I'm not very familiar with this
type of treatment. On sigma, collagenase IV is varied, as well as somewhat
expensive. I was wondering if anyone had tips on the standards for
ordering collagenase IV, as well as any protocols for this type of
treatment.
Thanks,
--Gregory Sliwoski
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