Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > C Elegans Forum
Register Search Today's Posts Mark Forums Read

C Elegans Forum C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.

sudan black staining

sudan black staining - C Elegans Forum

sudan black staining - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.

LinkBack Thread Tools Display Modes
Old 11-30-2005, 03:45 PM
Tim Schulz
Posts: n/a
Default sudan black staining

Dear all,
I am looking for a sudan black staining protocol that works for adult worms. So
far, I only got stained eggs in the worms, but I suspect this is not as it
should be?
many thanks,

Tim Schulz
University of Jena
Dept. of Human Nutrition
29, Dornburger Street
D-07743 Jena

+49(0)3641 949647
[Only registered users see links. ]

This mail was sent through [Only registered users see links. ]

Reply With Quote
Old 09-06-2013, 06:10 AM
Zagami's Avatar
Ph.D Doctorate
Points: 4,670, Level: 46 Points: 4,670, Level: 46 Points: 4,670, Level: 46
Activity: 0% Activity: 0% Activity: 0%
Join Date: Sep 2009
Location: Tabaka Mission Hospital - Kiisi -Kenya & Italy Sicily
Posts: 175
Thanks: 2
Thanked 28 Times in 24 Posts
Default Re: sudan black staining

Sudan Black adult worm stain is realized fixed worms in 1 ml of 1% paraformaldehyde in PBS by rocking at room temperature for 15 min, subjected to three freeze-thaw cycles, and dehydrated sequentially in three different concentrations of ethanol (25, 50, and 70%). The worms then are stained for 16 hrs in a 50% saturated solution of Sudan Black B (Sigma cod. S2380) in 70% ethanol, then rehydrated, and photographed.
Reply With Quote

black , staining , sudan

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
How are black holes created? LARGE HADRON COLLIDER hanson Physics Forum 2 09-09-2008 06:19 PM
What is Gravity? sdr@sdrodrian.com Physics Forum 1 01-27-2008 02:53 PM
How can a black hole "evaporate"? e goldstein Physics Forum 1 02-05-2005 12:58 AM
Information Can Leave A Black Hole James Briggs Physics Forum 4 12-18-2003 06:54 AM
Earth wobble related earthquake triggering Sept. 4, 2003 E.D.G. Physics Forum 1 09-05-2003 05:32 PM

All times are GMT. The time now is 01:23 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2015, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13464 seconds with 16 queries