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sudan black staining

sudan black staining - C Elegans Forum

sudan black staining - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


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  #1  
Old 11-30-2005, 03:45 PM
Tim Schulz
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Default sudan black staining





Dear all,
I am looking for a sudan black staining protocol that works for adult worms. So
far, I only got stained eggs in the worms, but I suspect this is not as it
should be?
many thanks,
Tim


Tim Schulz
University of Jena
Dept. of Human Nutrition
29, Dornburger Street
D-07743 Jena
Germany

+49(0)3641 949647
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Old 09-06-2013, 06:10 AM
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Default Re: sudan black staining

Sudan Black adult worm stain is realized fixed worms in 1 ml of 1% paraformaldehyde in PBS by rocking at room temperature for 15 min, subjected to three freeze-thaw cycles, and dehydrated sequentially in three different concentrations of ethanol (25, 50, and 70%). The worms then are stained for 16 hrs in a 50% saturated solution of Sudan Black B (Sigma cod. S2380) in 70% ethanol, then rehydrated, and photographed.
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