Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > C Elegans Forum
Register Search Today's Posts Mark Forums Read

C Elegans Forum C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


gene gun

gene gun - C Elegans Forum

gene gun - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 10-13-2003, 01:48 PM
Hoepfner
Guest
 
Posts: n/a
Default gene gun



With interest I read through the comments on your webpage about
Microparticle Bombardment Techniques. I wonder
if there is a real discussion forum out there somewhere about this
mettier? If you would like you could post the following
for me:

Hi everyone. I have used the gene gun PDS-1000 with a variety of
constructs. My finding was that small cytosolic
proteins work fine. With larger constructs I frequently see that the
unc-119
worms I am bombarding are rescued but there does not seem to be any
expression of the GFP-fusion protein. This
is especially frequent with larger constructs (>10kb). Since the
unc-119 recue gene is on the same plasmid as the
gene::GFP sequence, I wonder what happened? All plasmids have been
sequenced and should (to the best of my
knowledge) express the candidate gene. It would be nice to gather some
statistics about what constructs work fine and
what did not.
Sebastian Hoepfner
MPI-CBG
Dresden

---

Reply With Quote
Reply

Tags
gene , gun


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Human Cytome Project - Update 24 Jan. 2005 Peter Van Osta Cell Biology and Cell Culture 1 08-01-2010 02:18 PM
New Saccharomyces Sequences 11/27/04 Mike Cherry Yeast Forum 0 11-29-2004 12:39 AM
New Saccharomyces Sequences 09/08/04 SGD Sequences Yeast Forum 0 09-13-2004 10:07 PM
New Saccharomyces Sequences 08/11/04 SGD Sequences Yeast Forum 0 08-12-2004 12:26 AM
New Saccharomyces Sequences 05/19/04 SGD Sequences Yeast Forum 0 05-23-2004 04:06 PM


All times are GMT. The time now is 08:45 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.15886 seconds with 16 queries