Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > C Elegans Forum
Register Search Today's Posts Mark Forums Read

C Elegans Forum C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


worms on 24-well plates

worms on 24-well plates - C Elegans Forum

worms on 24-well plates - C Elegans Forum. Discuss the worm, wormbase, c. elegans, its culture and experimentation.


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-17-2003, 02:23 PM
abd elnasser
Guest
 
Posts: n/a
Default worms on 24-well plates



Dear Barth:
Sorry for replying your question late, it was alwaysin mind to reply but
there was no chance last days. It happened with me as well in the 24 plates
that the wells outside are drying faster. this is obviously becasue because
the wells outisde have more exposure to the (dry) air outside the plates.
so, all ideas that avoid that situation will help, as following for example:

1. while drying under the laminar flow, put some microliter od sterilized
water in all wells which are on the side of the side that te air is coming
from and you might add more, or better than that you might put always an
extra plates that have aonly water as a barier between your experimental
plates and the air. this will let the air coing over your plates having
almost the same amount of moisture. so, it will dry more in the same speed
(specially if you turn the plates from time to time).
2. with keeping the plates, might the same ideas of surrounding your
experimental wells with wells that contains just water, it depend on your
stack organization !. Parafilm arounf the plates and/or extra container with
water into your incubator might help to raise moisture level.
I would like as well to hear if you have got some crative idea replying
your question. good luck.
nasser

---
Reply With Quote
Reply

Tags
24well , plates , worms


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
PAGE gel sticked on both plates Patricia C Arbour-Reily Protocols and Methods Forum 0 12-08-2008 03:41 PM
Simultaneous bagging on 15 plates, N2 and 3 other strains Kathryn Hedges C Elegans Forum 0 11-12-2006 01:49 PM
'crusty food' solutions Ryder, Elizabeth F C Elegans Forum 0 01-11-2005 09:29 PM
responses to immobilizing live worms Hanna Fares C Elegans Forum 0 05-10-2004 08:18 PM
The Classical Vacuum - by Timothy H. Boyer Laurent Physics Forum 8 01-14-2004 03:49 PM


All times are GMT. The time now is 05:06 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11594 seconds with 16 queries