I'm a PhD student in plant molecular biology.
I'm trying to do an assay where i need to electroporate tomato
protoplasts and also test for reporter gene activity. I am really
running out of time and i need some immediate help
I'm able to get the protoplasts from both leaves and suspension cells
, but i am not able to keep them alive after electroporation. I'm
using ECM 830 (BTX) electroporator.
I need some information regarding the electroporation conditions for
tomato protoplasts using this machine and also GUS reporter gene assay
and anyother information that would keep my protoplasts alive and
uptake the DNA.
Any help in this regard will be very much appreciated.
Thank you in advance
|protoplast , transfection|
|Thread||Thread Starter||Forum||Replies||Last Post|
|HepG2 Cell Transfection||TMU||Transfection Forum||2||10-05-2009 02:38 PM|
|Any Transfection kit for HepG2 cells?||drkashyap||Transfection Forum||5||02-25-2009 10:10 PM|
|Transfection myoblast||laure d'astarac||Protocols and Methods Forum||0||09-24-2008 01:55 PM|
|Transfection Problem!||molecule2005||Transfection Forum||1||04-16-2008 09:15 AM|
|Cell Adhesion Problem After Transfection||gandalfthegrey||Cell Biology and Cell Culture||1||04-09-2008 02:40 PM|