Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > Molecular Research Topics Forum > Animal and Molecular Model Systems > Botany Forum
Register Search Today's Posts Mark Forums Read

Botany Forum Botany Forum


protoplast transfection

protoplast transfection - Botany Forum

protoplast transfection - Botany Forum


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-06-2004, 03:58 AM
Roopa
Guest
 
Posts: n/a
Default protoplast transfection



Hello colleagues

I'm a PhD student in plant molecular biology.
I'm trying to do an assay where i need to electroporate tomato
protoplasts and also test for reporter gene activity. I am really
running out of time and i need some immediate help

I'm able to get the protoplasts from both leaves and suspension cells
, but i am not able to keep them alive after electroporation. I'm
using ECM 830 (BTX) electroporator.

I need some information regarding the electroporation conditions for
tomato protoplasts using this machine and also GUS reporter gene assay
and anyother information that would keep my protoplasts alive and
uptake the DNA.

Any help in this regard will be very much appreciated.

Thank you in advance
Reply With Quote
Reply

Tags
protoplast , transfection


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
HepG2 Cell Transfection TMU Transfection Forum 2 10-05-2009 02:38 PM
Any Transfection kit for HepG2 cells? drkashyap Transfection Forum 5 02-25-2009 11:10 PM
Transfection myoblast laure d'astarac Protocols and Methods Forum 0 09-24-2008 01:55 PM
Transfection Problem! molecule2005 Transfection Forum 1 04-16-2008 09:15 AM
Cell Adhesion Problem After Transfection gandalfthegrey Cell Biology and Cell Culture 1 04-09-2008 02:40 PM


All times are GMT. The time now is 08:00 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10845 seconds with 16 queries