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Discuss hypotheses proposes for seedlessness inheritance in grapevine Hypotheses: Sato et. al (1994) ,5 dominat genes. Ledbetter and Burgos 1994 ,3 dominat genes Bouquet and Danglot 1996 ( vitis, 1996 35(1) :35-42), 3 recessive genes and 1 dominant regulator gene. What hypothese is the most satisfying or exist another molecular hypothese for explain stenospermocarpic on grapes? Ramón Pérez C. Lab de Biotecnología Inia , La platina Chile -- Ramón Pérez ------------------------------------------------------------------------ posted via [Only registered and activated users can see links. Click Here To Register...] ----== Posted via Newsfeed.Com - Unlimited-Uncensored-Secure Usenet News==---- [Only registered and activated users can see links. Click Here To Register...] The #1 Newsgroup Service in the World! >100,000 Newsgroups ---= 19 East/West-Coast Specialized Servers - Total Privacy via Encryption =--- |
Discuss hypotheses proposes for seedlessness inheritance in grapevine In article <[Only registered and activated users can see links. Click Here To Register...].uk>, [Only registered and activated users can see links. Click Here To Register...] [Ramón Pérez] wrote... Don't know [But why should all grape seedless variants necessarily have to be similar to one another? Weren't there multiple independent origins of seedless varieties, possibly involving different genes and different mutations?]. Anyway, it's not my area, but results of a quick Biological Abstracts database search include a few more recent citations-with-abstracts that may be of interest to you: Title: Genetic mapping of grapevine (Vitis vinifera L.) applied to the detection of QTLs for seedlessness and berry weight. Author, Editor, Inventor: Doligez-A {a}; Bouquet-A; Danglot-Y; Lahogue-F; Riaz-S; Meredith-C-P; Edwards-K-J; This-P Source: Theoretical-and-Applied-Genetics. [print] October, 2002; 105 (5): 780-795. Abstract: Parental and consensus genetic maps of Vitis vinifera L. (2n=38) were constructed using a F1 progeny of 139 individuals from a cross between two partially seedless genotypes. The consensus map contained 301 markers (250 amplification fragment length polymorphisms (AFLPs), 44 simple sequence repeats (SSRs), three isozymes, two random amplified polymorphic DNAs (RAPDs), one sequence-characterized amplified region (SCAR), and one phenotypic marker, berry color) mapped onto 20 linkage groups, and covered 1,002 cM. The maternal map consisted of 157 markers covering 767 cM (22 groups). The paternal map consisted of 144 markers covering 816 cM (23 groups). Differences in recombination rates between these maps and another unpublished map are discussed. The major gene for berry color was mapped on both the paternal and consensus maps. Quantitative trait loci (QTLs) for several quantitative subtraits of seedlessness in 3 successive years were searched for, based on parental maps: berry weight, seed number, seed total fresh and dry weights, seed percent dry matter, and seed mean fresh and dry weights. QTLs with large effects (R2 up to 51%) were detected for all traits and years at the same location on one linkage group, with some evidence for the existence of a second linked major QTL for some of them. For these major QTLs, differences in relative parental effects were observed between traits. Three QTLs with small effects (R2 from 6% to 11%) were also found on three other linkage groups, for berry weight and seed number in a single year, and for seed dry matter in 2 different years. Title: Breeding and genetic study on hybrid combinations between seeded and seedless grapevine (Vitis vinifera L.) cultivars. Author, Editor, Inventor: Roytchev-V {a} Source: Genetics-and-Breeding. [print] 2000; 30 (1-2): 35-47. Abstract: Through breeding and genetic investigations in F1 progeny of hybrid combinations between seeded and seedless grapevine cultivars, it was established that berry size, shape and weight are polymeric traits, inherited through incomplete dominance of cultivars with smaller and round berries. The phenotypic manifestation of seed weight and number in seeded forms confirmed that berry seedlessness is determined by dominant genes, too. To develop new high-yielding elongated seedless grapevine cultivars it is necessary to cross large-fruited parent cultivars with oblong berries, and selection should be done on a large number of plants. Title: A stenospermocarpic, seedless Vitis vinifera X Vitis rotundifolia hybrid developed by embryo rescue. Author, Editor, Inventor: Ramming-David-W {a}; Emershad-Richard-L; Tarailo-Ronald Source: Hortscience-. [print] July, 2000; 35 (4): 732-734. Abstract: Hybridizations between seedless Vitis vinifera L. genotypes and V. rotundifolia (Michx.) were made specifically to introgress the seedless trait into the disease-resistant background of V. rotundifolia. The seedless, gynoecious P79-101 was hybridized with three V. rotundifolia parents, producing a total of 102 ovules. From these ovules, 44 embryos developed, producing 20 plants. Isozyme analyses and morphological traits confirmed that 19 of the plants were hybrids. Sixteen were planted in an experimental vineyard at California State Univ. Fresno. One seedling, C41-5, produced seedless fruit that appeared to be stenospermocarpic based on fruit and aborted seed size. Fruit weight was slightly less than that of 'Thompson Seedless' (stenospermocarpic) and at least twice that of parthenocarpic fruit of 'Black Corinth' and C41-7, a seeded hybrid with many parthenocarpic fruit. Aborted seeds of C41-5 were larger than, but not significantly different from,those of 'Thompson Seedless', while parthenocarpic fruit from 'Black Corinth' and C41-7 had aborted seeds that were smaller than those of C41-5. Seed weight of C41-7 averaged almost 10 times that of aborted C41-5 seeds. This is the first report of a stenospermocarpic, seedless hybrid of V. vinifera X V. rotundifolia. Title: Identification of a codominant scar marker linker to the seedlessness character in grapevine. Author, Editor, Inventor: Lahogue-F {a}; This-P; Bouquet-A Source: Theoretical-and-Applied-Genetics. Oct., 1998; 97 (5-6) 950-959. Abstract: The variety Vitis vinifera cv Sultanine presents a type of seedlessness in which fertilization occurs but seeds subsequently fail to develop. It has been suggested that this trait might be controlled by three complementary recessive genes regulated by a dominant gene named I. Bulk segregant analysis was used to search for random amplified polymorphic DNA (RAPD) markers linked to the I gene in progeny obtained by crossing two partially seedless genotypes. One hundred and forty decamer primers were screened using bulks obtained by pooling the DNA of extreme individuals from the phenotypic distribution. We identified two RAPD markers which appeared tightly linked to I (at 0.7 and 3.5 cM respectively). The closest marker was used to develop a codominant SCAR (sequence characterized amplified region), named SCC8. This latter marker appeared of great value either to exclude from the progeny potentially seeded individuals or to select for seedless individuals. Indeed, all the seeded individuals of the progeny were found to be homozygous scc8-/scc8-,and all the individuals homozygous SCC8+/SCC8+ were seedless. Moreover, this marker was successfully applied to other natural seedless varieties where codominance persisted. SCC8 was also used to dissect more precisely the genetics of seedlessness. ANOVA analysis indicated that this SCAR marker accounted for at least 64.9% of the phenotypic variation of the seed's fresh weight and for at least 78.7% of the phenotypic variation of the seed's dry matter. These results confirmed the presence of a major gene, and also the existence of other complementary recessive genes, controlling the expression of seedlessness. Title: Influence of grape genotype, ripening season, seed trace size, and culture date on in ovule embryo development and plant formation. Author, Editor, Inventor: Pommer-Celso-V {a}; Ramming-David-W; Emershad-Richard-L Source: Bragantia-. 1995; 54 (2) 237-249. Abstract: Eighteen seedless grape genotypes differing in ripening season (early, mid and late) and in seed trace size (small, medium and large) were harvested at 6, 10, 14, 18 and 22 weeks past bloom (wpb). Using embryo rescue techniques it was studied if embryo do abort as the fruit matures and what percent embryos remain viable at later stages. The size of seed trace was also investigated to determine its influence on embryo viability during maturation. It was found that genotype have great influence on embryo culture traits. Late maturing genotypes showed fewer rescued embryos, germinated embryos and transplantable plants than early and mid season ones. The best culture time for grape embryo rescue is 6 and 10 wpb. At these dates, the largest number of embryos, germinated embryos and transplantable plants were obtained. Genotypes with the largest ratio for seed trace weight/seed trace length (i.e., largest density) showed the greatest tendency to have the largest number of ovules with embryos, more germinated embryos and more transplantable plants. The study also showed that it is possible to recover plants from mature fruit harvested late, although at a much reduced rate. Title: Identifying molecular genetic markers associated with seedlessness in grape. Author, Editor, Inventor: Striem-M-J; Ben-Hayyim-G; Spiegel-Roy-P {a} Source: Journal-of-the-American-Society-for-Horticultural-Science. 1996; 121 (5) 758-763. Abstract: Excluding seeded offspring at an early stage could be of great value to the breeder concerned with the development of seedless grapes (Vitis vinifera L.). We used the random amplified polymorphic DNA (RAPD) technique to identify molecular genetic markers, analyzing 82 individuals of a progeny resulting from a cross between 'Early Muscat' (seeded) and 'Flame Seedless'. Seven variables representing the traits of seedlessness were analyzed: mean fresh weight of one seed, total fresh weight of seeds per berry, perception of seed content, seed size categories evaluated visually, degree of hardness of the seed coat, degree of development of the endosperm, and degree of development of the embryo. Among 160 10mer primers, 110 gave distinct band patterns. Twelve markers yielded significant correlations with several subtraits of seedlessness, mainly with the mean fresh weight of one seed and the total fresh weight of seeds per berry. Multiple linear regression analysis resulted in high coefficients, such as R = 0.779 for fresh weight of seeds per berry, when the seven markers were included as independent variables in the model. Most of the seeded individuals, about 44% of the progeny, could be excluded using a two-step process of marker assisted selection. Title: Inheritance of stenospermocarpic seedlessness in Vitis vinifera L. Author, Editor, Inventor: Ledbetter-C-A; Burgos-L Source: Journal-of-Heredity. 1994; 85 (2) 157-160. Abstract: The inheritance of stenospermic seedlessness in Vitis vinifera L. was studied in F-1 populations obtained from seeded by seedless crosses. Two distinct types of stenospermic male parents were used. Male parents were obtained either from the progeny of seeded by seedless crosses or from in ovulo embryo cultures of seedless by seedless hybridizations. Based on the segregation ratios of progeny populations, an inheritance model for the stenospermic trait is proposed, consisting of three dominant complementary factors. Three independently inherited genes are necessary to produce a seedless phenotype. A homozygous recessive condition at any of the three loci would produce a seeded phenotype. Emphasis is placed on the high percentage of seedlessness obtained in crosses where the males used were derived from seedless by seedless progenies. Title: INHERITANCE STUDIES OF SEEDLESSNESS IN GRAPES. Author, Editor, Inventor: LOOMIS-N-H {a}; WEINBERGER-J-H Source: Journal-of-the-American-Society-for-Horticultural-Science. 1979; 104 (2): 181-184. Abstract: More than 10,000 seedlings were studied to elaborate the mode of inheritance of seedlessness in grapes (Vitis spp.). Self-pollinations of seeded selections having seedlessness in their parentage gave 0-10.7% seedless progeny. Crosses between seeded selections with seedlessness in 1 or both parents gave similar proportions. Crosses of seeded .times. seedless selections gave 0-55% seedless. Results were extremely variable, without apparent correlation to normal genetic ratios. Seedlessness appeared to be largely controlled by recessive factors. cheers |
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