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HEK clumping problem

Posted 11-18-2008 at 08:03 PM by suma_na

Hi,

I am working with HEK 293T cells, I got them from ATCC ,till 2 passages they were ok after that they are started to get clumped and they are not attaching well. I am trying to separate them by pipetting, but they are not
Anybody has any suggestion, I have few cells left in my flask now, can I still get them in monolayer.

help.

Sj

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Comments

1. 	hello do you trypsinize them well when subculturing? cheers
   	Posted 11-21-2008 at 01:37 AM by admin

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