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Prob with realtime pcr

Posted 01-05-2009 at 10:55 AM by nageswaran sivalingam

Hello,
If any one could answer my question i will be happy, What could be the reason for getting efficiency around 60% in my real time pcr, i am using SYBR green I dye. In the same run some sample showing 100% efficiency while most of other samples showing only 60-80% efficiency.


Hope i will get answers to my prob.

With love,
Nageswaran.S

Posted in Troubleshooting

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nageswaran sivalingam

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