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Hi All,
Prof. Dr. Mikael Kubista from TATAA Biocenter, Sweden is conducting qPCR related workshops in Bangalore, India - 1st Oct 08 for Students and 3rd Oct 08 for Industry/ Academic Delegates. Visit www.polyclonebio.com/workshops.html for registration details.
thanks,
Sanjay

Hi,

I am trying to compare the phosphorlyation profile in total cell lysates via western blot. Has anyone heard of using ERK as a housekeeping protein to normalize total protein loads? Many people use beta-actin, but this has not been working as one of my samples is negative for beta-actin.

Hi everybody, I work with DNA mismatch repair proteins of Arabidopsis and I have problems for its detection using western blot. So, I`ll try use immunoprecipitation with polyclonal antibodies. I you have interesting tips for this please let me know. Thanks!!!

Quantitative traits are naturally occurring variation, to some extent, they are difficult to identify. However, now three strategies have been developed to identify the quantitative traits of interest, QTL mapping, association mapping, and selection screening. All of them are related to genetic markers, so can you tell me how to identify a quantitative trait of interest using genetic markers as tools.