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-   -   Need appllications for my ph.d topic in gene screening (http://www.molecularstation.com/forum/biophysics-forum/73320-need-appllications-my-ph-d-topic-gene-screening.html)

harish 07-08-2010 09:44 AM

Need appllications for my ph.d topic in gene screening
 
Hallo all,
I am doing pcr for ssDNA transcripts of very short length directly from pcr like primer walking and it sort of works . I ran them on denaturing PAA-UREA gels. I also get multiple products when i chnage the primers.
then product length is very small. I want suggestions of further development and applications. We do this to screen different organisms on microarrays.
Also on microarrays i dont see any signal when i dilute the pcr product (which has mutiple ssDNA transcripts). Does western blot work for this.
Sometimes i doubt if this is not a novel one and can be done very easily.
Thanks in advance,
Harish.


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