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#1
08-26-2008, 09:36 PM
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 enriching for DNA palindromes Does anyone have any idea how to go about enriching for DNA palindrome structures from genomic DNA? The way I have been doing it is: - cut up whole genome with restriction enzymes - denaturing with boiling and high salt - allowing the arms to "snap-back" on each other with rapid cooling - digesting away single stranded DNA with S1 nuclease but I'm still not getting the enrichment I want, or maybe too much single stranded DNA contamination. Any ideas are greatly appreciated, thank you!!  |
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| dna , enriching , palindromes |
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