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enriching for DNA palindromes
Does anyone have any idea how to go about enriching for DNA palindrome structures from genomic DNA?
The way I have been doing it is:
- cut up whole genome with restriction enzymes
- denaturing with boiling and high salt
- allowing the arms to "snap-back" on each other with rapid cooling
- digesting away single stranded DNA with S1 nuclease
but I'm still not getting the enrichment I want, or maybe too much single stranded DNA contamination.
Any ideas are greatly appreciated, thank you!!
|dna , enriching , palindromes|
|Thread||Thread Starter||Forum||Replies||Last Post|
|enriching for DNA palindromes??||neoserenity333||DNA Techniques||2||08-27-2008 05:11 AM|