Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


DIY spray dry EDTA onto pipette tips?

DIY spray dry EDTA onto pipette tips? - Biology Forum

DIY spray dry EDTA onto pipette tips? - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-20-2014, 10:49 AM
Pipette Filler
Points: 379, Level: 7 Points: 379, Level: 7 Points: 379, Level: 7
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2014
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Question DIY spray dry EDTA onto pipette tips?



Hi,

I'm trying to collect zebrafish blood for RNA analysis. We plan to pool blood from 6-10 animals to get the necessary volume (~500 uL), but my question is about how to prevent coagulation of the blood. Obviously, the pre-coated tubes that are commercially available are too large for my purpose. So I think given the small volume that it would be ideal to have the EDTA (or other anti-coagulant- Sodium citrate, heparin, etc) on the pipette tip that the blood is collected with, the blood can easily be pipetted up and down a few times to ensure good mixing with this method, as well.

But I don't know how to make these myself. We own powdered EDTA (I think the typical used is K2EDTA), and the tips, although we can buy anything needed. Is it just as simple as making a liquid of dissolved EDTA and water then coating the inside and letting dry? If so what concentration of solution? These would be 200 uL non-barrier tips that I am working with.

Thanks in advance!
Niki
Reply With Quote
  #2  
Old 02-24-2014, 01:16 AM
butters's Avatar
M.D/Ph.D
Points: 4,938, Level: 47 Points: 4,938, Level: 47 Points: 4,938, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2008
Location: Malaysia
Posts: 315
Thanks: 25
Thanked 68 Times in 63 Posts
Default Re: DIY spray dry EDTA onto pipette tips?

I guess the biggest problem would be how sure are you all the EDTA will be fully dissolve by the time you aspirate up and down?

Why not just aliquot require EDTA on smaller tube in house?
Reply With Quote
  #3  
Old 02-25-2014, 09:23 AM
Pipette Filler
Points: 379, Level: 7 Points: 379, Level: 7 Points: 379, Level: 7
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2014
Posts: 8
Thanks: 0
Thanked 0 Times in 0 Posts
Default Re: DIY spray dry EDTA onto pipette tips?

I ended up finding a protocol online for collecting blood using EDTA

I'm not allowed to post links until I've posted a little more, but if you do a google search for "Blood Collection for EIAs" it's the first result
Reply With Quote
  #4  
Old 02-26-2014, 12:55 AM
butters's Avatar
M.D/Ph.D
Points: 4,938, Level: 47 Points: 4,938, Level: 47 Points: 4,938, Level: 47
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Dec 2008
Location: Malaysia
Posts: 315
Thanks: 25
Thanked 68 Times in 63 Posts
Default Re: DIY spray dry EDTA onto pipette tips?

cool than you did manage to find a solution
Reply With Quote
Reply

Tags
diy , dry , edta , pipette , spray , tips


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
weight loss tips!!! Mart Instructions for Posting, Help, and Frequently Asked Questions 3 09-13-2014 09:18 AM
Volumetric Pipette: helan General Science Questions and Layperson Board 0 06-05-2012 05:26 AM
Stability of EDTA? Satoshi Chemistry Forum 1 11-13-2003 01:52 PM


All times are GMT. The time now is 09:05 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.13321 seconds with 16 queries