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-   -   DIY spray dry EDTA onto pipette tips? (http://www.molecularstation.com/forum/biology-forum/88167-diy-spray-dry-edta-onto-pipette-tips.html)

Niki24601 02-20-2014 10:49 AM

DIY spray dry EDTA onto pipette tips?
 
Hi,

I'm trying to collect zebrafish blood for RNA analysis. We plan to pool blood from 6-10 animals to get the necessary volume (~500 uL), but my question is about how to prevent coagulation of the blood. Obviously, the pre-coated tubes that are commercially available are too large for my purpose. So I think given the small volume that it would be ideal to have the EDTA (or other anti-coagulant- Sodium citrate, heparin, etc) on the pipette tip that the blood is collected with, the blood can easily be pipetted up and down a few times to ensure good mixing with this method, as well.

But I don't know how to make these myself. We own powdered EDTA (I think the typical used is K2EDTA), and the tips, although we can buy anything needed. Is it just as simple as making a liquid of dissolved EDTA and water then coating the inside and letting dry? If so what concentration of solution? These would be 200 uL non-barrier tips that I am working with.

Thanks in advance!
Niki

butters 02-24-2014 01:16 AM

Re: DIY spray dry EDTA onto pipette tips?
 
I guess the biggest problem would be how sure are you all the EDTA will be fully dissolve by the time you aspirate up and down?

Why not just aliquot require EDTA on smaller tube in house?

Niki24601 02-25-2014 09:23 AM

Re: DIY spray dry EDTA onto pipette tips?
 
I ended up finding a protocol online for collecting blood using EDTA

I'm not allowed to post links until I've posted a little more, but if you do a google search for "Blood Collection for EIAs" it's the first result

butters 02-26-2014 12:55 AM

Re: DIY spray dry EDTA onto pipette tips?
 
cool than you did manage to find a solution


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