Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biology Forum
Register Search Today's Posts Mark Forums Read

Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Bodipy-FL

Bodipy-FL - Biology Forum

Bodipy-FL - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-19-2014, 08:22 PM
Pipette Filler
Points: 54, Level: 1 Points: 54, Level: 1 Points: 54, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2014
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Bodipy-FL



Hi!

I am currently working with CHO cells trying to optimize Bodipy-FL dye for FACs. Has anyone worked with this dye before for FACs analysis? I understand that it fluoresces green when in the endoplasmic reticulum and should shift to red when in the Golgi. I am getting a decents red signal, however, I am not noticing a decrease in the green signal (even when I allow a chase time upto 48 hours). Any advice, help, literature would be greatly appreciated!


Thanks!
Budding Scientist
Reply With Quote
Reply


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump


All times are GMT. The time now is 03:50 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.10643 seconds with 15 queries