I am currently working with CHO cells trying to optimize Bodipy-FL dye for FACs. Has anyone worked with this dye before for FACs analysis? I understand that it fluoresces green when in the endoplasmic reticulum and should shift to red when in the Golgi. I am getting a decents red signal, however, I am not noticing a decrease in the green signal (even when I allow a chase time upto 48 hours). Any advice, help, literature would be greatly appreciated!
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