Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Biology Forum (http://www.molecularstation.com/forum/biology-forum/)
-   -   Site directed mutagenesis (http://www.molecularstation.com/forum/biology-forum/88016-site-directed-mutagenesis.html)

jayesh desale 07-29-2013 10:12 AM

Site directed mutagenesis
 
i have read your query about pcr mutagenesis.
Even i am facing the same problem in Site directed mutagenesis.
After getting colonies, I am not able to isolate plasmid from XL1-Blue transformed supercompetent cells and even not able to transform it further in DH5a E.coli cells.
Please suggest me as i have stucked in between and not able to give samples for sequencing.


All times are GMT. The time now is 11:59 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.08787 seconds with 11 queries