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Regarding protein isolation from drosophila head and wester blotting

Regarding protein isolation from drosophila head and wester blotting - Biology Forum

Regarding protein isolation from drosophila head and wester blotting - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 06-10-2013, 12:29 PM
Pipette Filler
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Default Regarding protein isolation from drosophila head and wester blotting



I am working on Alzheimer's disease using drosophila model system.
I want to isolate the protein from drosophila head for western blotting for amyloid beta protein. so which homogenizing buffer is best to isolate protein from drosophila head in respect of amyloid beta protein.
and what should be the condition for the running SDS-PAGE including percentage of gel and transfer condition.
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