Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Biology Forum (http://www.molecularstation.com/forum/biology-forum/)
-   -   My purified protein was degraded but had high activity (http://www.molecularstation.com/forum/biology-forum/87824-my-purified-protein-degraded-but-had-high-activity.html)

godfinger 04-25-2013 06:13 AM

My purified protein was degraded but had high activity
 
I expressed a recombination protein in Pichia pastoris and got pure protein after purified from Ni-column. I confirmed my pure protein was only one band in SDS-PAGE, however, it was degraded after storing in few days. The molecular weight of the degraded protein was decrease about 10kDa. It is interesting that degraded protein had better activity than undergrad one. According
to this result, is there any thing that can degrades my pure protein or my protein has auto-degradation? In anyone can help me found out these question?
:notworthy:
My protein is glycohydrolase, not protease.


All times are GMT. The time now is 01:52 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09075 seconds with 11 queries