40-50 pcr cycles and Ct-values of 36-38 - trust or not?
I was wondering, if you run 40 pcr cycles, are Ct-vales between 36-40 meningful or not?
And, 50 pcr cycles are useful to find mRNA expression? This is correct?
I was looking PRL in culture cells, but Ct values were between 36-38; and even in my controls I didnīt obtain Ct values. What happend? Please, somebody help me!!
Can somebody give me any reference dealing with these topics?
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