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-   -   40-50 pcr cycles and Ct-values of 36-38 - trust or not? (http://www.molecularstation.com/forum/biology-forum/87782-40-50-pcr-cycles-ct-values-36-38-trust-not.html)

Pix 04-11-2013 04:34 AM

40-50 pcr cycles and Ct-values of 36-38 - trust or not?
 
Hello!!

I was wondering, if you run 40 pcr cycles, are Ct-vales between 36-40 meningful or not?

And, 50 pcr cycles are useful to find mRNA expression? This is correct?

I was looking PRL in culture cells, but Ct values were between 36-38; and even in my controls I didnīt obtain Ct values. What happend? Please, somebody help me!!
Can somebody give me any reference dealing with these topics?

Sincerely,

Pix


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