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Conditional knockout mouse
I am using transgenic mouse where my gene of interest is floxed. When I cross this mouse line with Tamoxifen inducible Cre line, CAGGCre-ERTM, I get mice with with heterozygous Cre and floxed gene of interest. The experimental plan was to isolate myoblasts from these mice and induce Cre in cell culture by addition of Tamoxifen. To my surprise, the cells isolated from these mice die with in few days, where as the cells from WT or Flox/flox mice do not. Could anyone tell me what could be the problem here and also give me some suggestions on how to bypass this problem.
Re: Conditional knockout mouse
DiART ( <a href="http://www.protein2.com">www.protein2.com</a> ), Deuterium Isobaric Amine Reactive isobaric Tags which share the amine-reactive chemistry used by iTRAQ and TMT that synthesize amine-derivatized peptides from protein digests.
The innovative advantages:
1) DiART-labeled peptides are chromatographically identical.
2) Have much stronger low-mass reporter ions than that of iTRAQ abd TMT.
3) Low price, at least 50% lower than iTRAQ and TMT
|conditional , inducible , knockout , knockout or transgenic , mouse|
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