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Bacterial cell disruption by using Lysozyme is failed!

Bacterial cell disruption by using Lysozyme is failed! - Biology Forum

Bacterial cell disruption by using Lysozyme is failed! - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 02-11-2013, 02:17 PM
Pipette Filler
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Question Bacterial cell disruption by using Lysozyme is failed!



Hi all,

I am going to extract RNA by using RNasy Midi kit,QIAGEN from a pseudomonas. The problem is that after adding TE buffer containing Lysozyme and centrifuge, there is no jelly pellet! While it is too viscose.
I tried to:
1- Dilute the solution a bit
2- increasing the time and g force
3- Taking less starting material

But still I get no pellet! I have done it once two days ago and got the pellet but not now! after destroying some samples!

Thank you in advance
Student1
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