|Register||Search||Today's Posts||Mark Forums Read|
|Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!|
| ||LinkBack||Thread Tools||Display Modes|
Bacterial cell disruption by using Lysozyme is failed!
I am going to extract RNA by using RNasy Midi kit,QIAGEN from a pseudomonas. The problem is that after adding TE buffer containing Lysozyme and centrifuge, there is no jelly pellet! While it is too viscose.
I tried to:
1- Dilute the solution a bit
2- increasing the time and g force
3- Taking less starting material
But still I get no pellet! I have done it once two days ago and got the pellet but not now! after destroying some samples!
Thank you in advance
|bacterial , cell , disruption , failed , lysozyme|
|Thread||Thread Starter||Forum||Replies||Last Post|
|Human Cytome Project - Update 24 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||1||08-01-2010 02:18 PM|
|Human Cytome Project - an idea - Update 19 April 2005||Peter Van Osta||Cell Biology and Cell Culture||1||06-01-2009 02:17 PM|
|A Human Cytome Project - an idea - Update 14 March 2005||Peter Van Osta||Cell Biology and Cell Culture||0||03-14-2005 01:27 PM|
|Human Cytome Project - Update 6 Jan. 2005||Peter Van Osta||Cell Biology and Cell Culture||0||01-06-2005 10:18 AM|
|Tissue Engineering & Stem Cell Research 2005||Andrew Oliver||Protocols and Methods Forum||0||12-02-2004 10:38 PM|