Bacterial cell disruption by using Lysozyme is failed!
I am going to extract RNA by using RNasy Midi kit,QIAGEN from a pseudomonas. The problem is that after adding TE buffer containing Lysozyme and centrifuge, there is no jelly pellet! While it is too viscose.
I tried to:
1- Dilute the solution a bit
2- increasing the time and g force
3- Taking less starting material
But still I get no pellet! I have done it once two days ago and got the pellet but not now! after destroying some samples!
Thank you in advance
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