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Problems Cloning Into TOPO ENTRE

Problems Cloning Into TOPO ENTRE - Biology Forum

Problems Cloning Into TOPO ENTRE - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 02-07-2013, 06:47 PM
Pipette Filler
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Default Problems Cloning Into TOPO ENTRE



Hi,

I wanted to know if somebody had cloned into a donor vector, after cloning in TOPO ENTRE, a gene that have the sequence CACC, and after a CA nucleotide and then the ATG. I mean: Do you obtained a good traduction of the protein? and the efficiency of the traduction was good too? What problems could I have and how I must carry out the experiments to resolve the possible problems?
Thanks a lot!
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