| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here! |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
02-07-2013, 05:47 PM
| |||||||||||
| |||||||||||
 Problems Cloning Into TOPO ENTRE Hi, I wanted to know if somebody had cloned into a donor vector, after cloning in TOPO ENTRE, a gene that have the sequence CACC, and after a CA nucleotide and then the ATG. I mean: Do you obtained a good traduction of the protein? and the efficiency of the traduction was good too? What problems could I have and how I must carry out the experiments to resolve the possible problems? Thanks a lot!   |
|
| Tags |
| cloning , entre , problems , topo |
| Thread Tools | |
| Display Modes | |
|
|
Similar Threads | ||||
| Thread | Thread Starter | Forum | Replies | Last Post |
| Directional TOPO Cloning | kdawg | Molecular Cloning Forum | 6 | 04-04-2013 11:42 PM |
| problems with topo ta cloning | ihatetopo | Molecular Cloning Forum | 2 | 10-19-2010 11:40 PM |
| TOPO cloning problems! | annabcn | Molecular Cloning Forum | 1 | 09-22-2009 07:04 AM |
| TOPO TA cloning | temschen | Molecular Cloning Forum | 1 | 02-06-2009 12:39 PM |
| topo ta cloning | as | Protocols and Methods Forum | 9 | 12-31-2004 06:06 PM |
Linear Mode
