Problems Cloning Into TOPO ENTRE
I wanted to know if somebody had cloned into a donor vector, after cloning in TOPO ENTRE, a gene that have the sequence CACC, and after a CA nucleotide and then the ATG. I mean: Do you obtained a good traduction of the protein? and the efficiency of the traduction was good too? What problems could I have and how I must carry out the experiments to resolve the possible problems?
Thanks a lot! :)
|All times are GMT. The time now is 10:53 AM.|
Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved