Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum

Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum (http://www.molecularstation.com/forum/)
-   Biology Forum (http://www.molecularstation.com/forum/biology-forum/)
-   -   Problems Cloning Into TOPO ENTRE (http://www.molecularstation.com/forum/biology-forum/87496-problems-cloning-into-topo-entre.html)

Flac@2013 02-07-2013 06:47 PM

Problems Cloning Into TOPO ENTRE
 
Hi,

I wanted to know if somebody had cloned into a donor vector, after cloning in TOPO ENTRE, a gene that have the sequence CACC, and after a CA nucleotide and then the ATG. I mean: Do you obtained a good traduction of the protein? and the efficiency of the traduction was good too? What problems could I have and how I must carry out the experiments to resolve the possible problems?
Thanks a lot! :)


All times are GMT. The time now is 07:49 AM.

Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved

Page generated in 0.09140 seconds with 11 queries