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second band on top of the free DNA template in EMSA
I performed an EMSA assay in which I used as a negative control only labeled DNA template not incubated with nuclear extract. The second sample has been incubated with nuclear extract of HeLa cells.
I can see two bands in my negative control, but canīt see is in my sample. Does anybody know what my second band is?
Thank you very much!
|band , dna , emsa , free , template , top|
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