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second band on top of the free DNA template in EMSA

second band on top of the free DNA template in EMSA - Biology Forum

second band on top of the free DNA template in EMSA - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 01-29-2013, 12:47 PM
Pipette Filler
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Default second band on top of the free DNA template in EMSA



Hello everybody

I performed an EMSA assay in which I used as a negative control only labeled DNA template not incubated with nuclear extract. The second sample has been incubated with nuclear extract of HeLa cells.
I can see two bands in my negative control, but canīt see is in my sample. Does anybody know what my second band is?

Thank you very much!
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