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-   -   second band on top of the free DNA template in EMSA (http://www.molecularstation.com/forum/biology-forum/87441-second-band-top-free-dna-template-emsa.html)

Slyminator 01-29-2013 12:47 PM

second band on top of the free DNA template in EMSA
 
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Hello everybody :wave:

I performed an EMSA assay in which I used as a negative control only labeled DNA template not incubated with nuclear extract. The second sample has been incubated with nuclear extract of HeLa cells.
I can see two bands in my negative control, but canīt see is in my sample. Does anybody know what my second band is?

Thank you very much!


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