| |||||||
| Register | Search | Today's Posts | Mark Forums Read |
| Biology Forum Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here! |
 |
| | LinkBack | Thread Tools | Display Modes |
|
#1
01-23-2013, 04:16 PM
| |||||||||||
| |||||||||||
 Colabelling for Immunofluorescence Hello, I want to do a co-labelling immunofluorescence experiment on mouse parafin-embedded tissue sections. The first primary antibody is a Anti-X raised in rabbit, IgG fraction and the second primary is Anti-Y raised in mouse. Since I know there can be issues with background staining using mouse monoclonal antibodies in mouse tissues, I'm not sure how I can account for this in my double staining protocol. I have optimized the protocol for Anti-X using a goat anti-rabbit secondary but haven't tried the mouse Anti-Y antibody yet since the tissue sections I have are valuable and can't be wasted. Can someone help with ideas/protocol to double stain with these primaries?  |
|
#2
01-23-2013, 04:38 PM
| |||||||||||
| |||||||||||
 ugg ブーツ ÿþ[ ÿþ[ ÿþ[ |
|
#3
01-23-2013, 04:45 PM
| |||||||||||
| |||||||||||
| ugg ムートン ブーツ ÿþ[ ÿþ[ ÿþ[ |
|
#4
01-23-2013, 04:53 PM
| |||||||||||
| |||||||||||
| アグ ブーツ ÿþ[ ÿþ[ ÿþ[ |
|
#5
01-29-2013, 05:28 AM
| |||||||||||
| |||||||||||
| Re: Colabelling for Immunofluorescence hello! i am a new member of this forum. Thanks, |
|
#6
04-02-2013, 08:42 PM
| |||||||||||
| |||||||||||
| Re: Colabelling for Immunofluorescence Hello arielwright111, any idea on troubleshooting this problem? Thanks, |
|
| Tags |
| antibody problems , colabeling , colabelling , immunofluorescence , mouse on mouse staining |
| Thread Tools | |
| Display Modes | |
|
|
Linear Mode
