I am carrying out isozyme analysis for glucose phosphate isomerase using cellulose acetate electrophoresis. The electrophoresis unit is Cellogel (Chemetron, Italy). Instead of producing bands that can be resolved, the whole cellulose acetate membrane is being stained. Parameters used for electrophoresis in the trials are
• 200 V, 10 mA, 60 mins
• 200 V, 50 mA, 30 mins.
The sample volume applied is 0.9 microlitres.
The pore size of the cellulose acetate membrane is 250 microns.
What could be the source of this problem?