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Isozyme analysis of Phytophthora infestans

Isozyme analysis of Phytophthora infestans - Biology Forum

Isozyme analysis of Phytophthora infestans - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 01-15-2013, 02:23 PM
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Default Isozyme analysis of Phytophthora infestans



I am carrying out isozyme analysis for glucose phosphate isomerase using cellulose acetate electrophoresis. The electrophoresis unit is Cellogel (Chemetron, Italy). Instead of producing bands that can be resolved, the whole cellulose acetate membrane is being stained. Parameters used for electrophoresis in the trials are
200 V, 10 mA, 60 mins
200 V, 50 mA, 30 mins.
The sample volume applied is 0.9 microlitres.
The pore size of the cellulose acetate membrane is 250 microns.
What could be the source of this problem?
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Old 01-16-2013, 02:14 AM
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Old 01-16-2013, 02:17 AM
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Old 01-16-2013, 04:52 AM
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Old 01-16-2013, 10:05 PM
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