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katy02 01-15-2013 02:23 PM

Isozyme analysis of Phytophthora infestans
I am carrying out isozyme analysis for glucose phosphate isomerase using cellulose acetate electrophoresis. The electrophoresis unit is Cellogel (Chemetron, Italy). Instead of producing bands that can be resolved, the whole cellulose acetate membrane is being stained. Parameters used for electrophoresis in the trials are
200 V, 10 mA, 60 mins
200 V, 50 mA, 30 mins.
The sample volume applied is 0.9 microlitres.
The pore size of the cellulose acetate membrane is 250 microns.
What could be the source of this problem?

Annabelledsd 01-16-2013 02:14 AM

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