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Recently i have started using TFA inactivation method to identify Cronobacter isolates as prescribed by many authors. however i have one problem with this method. my sample do not stay on the spot and tend to move from one spot to another if i don't hold it with pipette till it is dried and it could be due to Hydrophilicity . do you have any solution, or correction. i have strictly followed protocol from Bruker daltonics.
Re: Maldi-tof ms
DiART ( <a href="http://www.protein2.com">www.protein2.com</a> ), Deuterium Isobaric Amine Reactive isobaric Tags which share the amine-reactive chemistry used by iTRAQ and TMT that synthesize amine-derivatized peptides from protein digests.
The innovative advantages:
1) DiART-labeled peptides are chromatographically identical.
2) Have much stronger low-mass reporter ions than that of iTRAQ abd TMT.
3) Low price, at least 50% lower than iTRAQ and TMT
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