Recently i have started using TFA inactivation method to identify Cronobacter isolates as prescribed by many authors. however i have one problem with this method. my sample do not stay on the spot and tend to move from one spot to another if i don't hold it with pipette till it is dried and it could be due to Hydrophilicity . do you have any solution, or correction. i have strictly followed protocol from Bruker daltonics.
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