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gel purification

gel purification - Biology Forum

gel purification - Biology Forums. Ask questions and discuss the study of Biology. If you have Biology questions from your homework ask them here!


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Old 01-01-2013, 09:38 AM
Pipette Filler
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Default gel purification



hiii frens
I am tryning to purify my desired amplicon (size 885 bp) from gel. I am using qiaquick gel extraction kit for this,but not getting good yeil and good 260/280 ratio when i analyse my sample on nanodrop after purification i want to use this sample for in vitro transcription.
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Old 03-28-2013, 05:40 AM
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Default Re: gel purification

Try re-eluting the column with half the volume of elution buffer.

Last edited by ajithnandanam; 03-28-2013 at 05:41 AM.
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